Illegal ingredients found in traditional Chinese medicines

For the first time, researchers at Murdoch University have used new DNA sequencing technology to reveal the animal and plant composition of traditional Chinese medicines (TCM) – with worrying results.

Research leader and Murdoch University Australian Research Council Future Fellow, Dr Mike Bunce, said that some TCMs contained potentially toxic plant ingredients, allergens and even traces of endangered animals.

“TCMs have a long cultural history but today, consumers need to be aware of the legal and health safety issues before adopting them as a treatment option,” Dr Bunce said.

Fifteen TCM samples, seized by border officials, in the form of powders, tablets, capsules, flakes and herbal teas were audited using the DNA preserved in the samples. The results are published in the journal PLoS Genetics.

“In total we found 68 different plant families in the medicines – they are complex mixtures of species,” Dr Bunce said.

“Some of the TCMs contained plants of the genus Ephedra and Asarum. These plants contain chemicals which can be toxic if the wrong dosage is taken, but none of them actually listed concentrations on the packaging”.

“We also found traces from trade restricted animals that are classified as vulnerable, endangered, or critically endangered, including the Asiatic black bear and Saiga antelope.”

Until now it has been difficult to determine the biological origins of ingredients contained within TCMs because processing into pills and powders makes identification difficult.

PhD student Megan Coghlan, who is studying the application of DNA techniques in wildlife forensic applications, said the research shows that second-generation, high throughput sequencing is an efficient and cost-effective way to audit the species composition.

“The approach has the ability to unravel complex mixtures of plant and animal products,” Ms Coghlan said.

Further testing of TCMs would reveal the extent of the problem and make it easier for custom officials to identify the trade of endangered species. The increasing popularity of the medicines has seen the value of the industry increase to hundreds of millions of dollars per annum – not good news for rare animals that are hunted and killed for use in TCMs.

“We found multiple samples that contained DNA from animals listed as trade-restricted according to the Convention on International Trade in Endangered Species Legislation. Put simply, these TCMs are illegal,” Ms Coglan said.

Another worrying concern is the mislabelling of TCMs meaning consumers are unaware of the presence of some ingredients including animal DNA and potentially allergens such as soy or nuts.

“A product labelled as 100 per cent Saiga antelope contained considerable quantities of goat and sheep DNA,” Dr Bunce said.

“Another product, Mongnan Tianbao pills, contained deer and cow DNA, the latter of which may violate religious or cultural strictures.”

Incorrect labelling makes it difficult to enforce legislation and to prosecute cases of illegal trade.

“It is hoped that this new approach to genetically audit medicinal products will bring about a new level of regulation to the area of complementary and alternative medicine,” Dr Bunce said.

“Auditing TCMs would assist in prosecuting individuals who seek to profit from the illegal trade in animal products.”

(Source: Murdoch University: PLoS Genetics)